Construction and characterization of a bacterial clone containing the hemagglutinin gene of the WSN strain (H0N1) of influenza virus
Identifieur interne : 002819 ( Main/Exploration ); précédent : 002818; suivant : 002820Construction and characterization of a bacterial clone containing the hemagglutinin gene of the WSN strain (H0N1) of influenza virus
Auteurs : Alan R. Davis [États-Unis] ; Alan L. Hiti [États-Unis] ; Debi P. Nayak [États-Unis]Source :
- Gene [ 0378-1119 ] ; 1980.
English descriptors
- Teeft :
- Acad, Agarose, Alkaline, Alkaline agarose, Amino, Amino acid sequence, Amino acid sequences, Amino acids, Cdna, Choppin, Crna, Dodecadeoxynucleotide primers, Fowl, Fowl plague virus, Gene, Hemagglutinin, Hon1, Influenza, Influenza virus, Lazarowitz, Natl, Nayak, Neutral agarose, Nick translation, Nuclease, Nucleotide, Nucleotide sequence, Other hon1 viruses, Other influenza, Palese, Peptide, Plasmid, Polymerase, Primer, Proc, Protein sequencing, Proteolytic cleavage, Psti, Reaction mixture, Sequencing, Sodium dodecyl sulfate, Untranslated region, Viral, Virology, Virus, Vrna, Vrna crna.
Abstract
Abstract: A synthetic dodecadeoxynucleotide primer has been used to prepare a double-stranded DNA form of the hemagglutinin (HA) gene of a human influenza virus (WSN strain, HON1). This DNA has been inserted in plasmid pBR322 and cloned in bacterial cells. The insert contains nearly the complete hemagglutinin gene. A restriction map of this insert has been determined and structurally important areas of the HA gene have been sequenced. Amino acid sequences of several regions of the HA protein were deduced from the DNA sequences and compared to the known amino acid sequences of other influenza A viruses. WSN HA shows extensive homology to all influenza A viruses in a few regions, namely the first 17 amino acids of the N-terminus of HA1 (N-terminal polypeptide of HA) and the first 24 amino acids of the N-terminus of HA2 (C-terminal polypeptide of HA). The sequence diverges extensively from other influenza A viruses in most other areas. The sequence of WSN virus HA is similar to that of other H0N1 viruses with the exception of the C-terminus of the HA1 peptide. The change in this area may contribute to some of the unique properties of WSN virus among the HON1 viruses. In addition, WSN HA contains a 17-amino-acid precursor before the N-terminus of HA1 and a single amino acid, arginine, connecting HA1 and HA2.
Url:
DOI: 10.1016/0378-1119(80)90050-5
Affiliations:
Links toward previous steps (curation, corpus...)
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- to stream Istex, to step Curation: 000F27
- to stream Istex, to step Checkpoint: 001433
- to stream Main, to step Merge: 002966
- to stream Main, to step Curation: 002819
Le document en format XML
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Nayak</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Microbiology and Immunology, UCLA School of Medicine, Los Angeles</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Gene</title><title level="j" type="abbrev">GENE</title><idno type="ISSN">0378-1119</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1980">1980</date><biblScope unit="volume">10</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="205">205</biblScope><biblScope unit="page" to="218">218</biblScope></imprint><idno type="ISSN">0378-1119</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0378-1119</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acad</term><term>Agarose</term><term>Alkaline</term><term>Alkaline agarose</term><term>Amino</term><term>Amino acid sequence</term><term>Amino acid sequences</term><term>Amino acids</term><term>Cdna</term><term>Choppin</term><term>Crna</term><term>Dodecadeoxynucleotide primers</term><term>Fowl</term><term>Fowl plague virus</term><term>Gene</term><term>Hemagglutinin</term><term>Hon1</term><term>Influenza</term><term>Influenza virus</term><term>Lazarowitz</term><term>Natl</term><term>Nayak</term><term>Neutral agarose</term><term>Nick translation</term><term>Nuclease</term><term>Nucleotide</term><term>Nucleotide sequence</term><term>Other hon1 viruses</term><term>Other influenza</term><term>Palese</term><term>Peptide</term><term>Plasmid</term><term>Polymerase</term><term>Primer</term><term>Proc</term><term>Protein sequencing</term><term>Proteolytic cleavage</term><term>Psti</term><term>Reaction mixture</term><term>Sequencing</term><term>Sodium dodecyl sulfate</term><term>Untranslated region</term><term>Viral</term><term>Virology</term><term>Virus</term><term>Vrna</term><term>Vrna crna</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A synthetic dodecadeoxynucleotide primer has been used to prepare a double-stranded DNA form of the hemagglutinin (HA) gene of a human influenza virus (WSN strain, HON1). This DNA has been inserted in plasmid pBR322 and cloned in bacterial cells. The insert contains nearly the complete hemagglutinin gene. A restriction map of this insert has been determined and structurally important areas of the HA gene have been sequenced. Amino acid sequences of several regions of the HA protein were deduced from the DNA sequences and compared to the known amino acid sequences of other influenza A viruses. WSN HA shows extensive homology to all influenza A viruses in a few regions, namely the first 17 amino acids of the N-terminus of HA1 (N-terminal polypeptide of HA) and the first 24 amino acids of the N-terminus of HA2 (C-terminal polypeptide of HA). The sequence diverges extensively from other influenza A viruses in most other areas. The sequence of WSN virus HA is similar to that of other H0N1 viruses with the exception of the C-terminus of the HA1 peptide. The change in this area may contribute to some of the unique properties of WSN virus among the HON1 viruses. In addition, WSN HA contains a 17-amino-acid precursor before the N-terminus of HA1 and a single amino acid, arginine, connecting HA1 and HA2.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Davis, Alan R" sort="Davis, Alan R" uniqKey="Davis A" first="Alan R." last="Davis">Alan R. Davis</name></region><name sortKey="Hiti, Alan L" sort="Hiti, Alan L" uniqKey="Hiti A" first="Alan L." last="Hiti">Alan L. Hiti</name><name sortKey="Nayak, Debi P" sort="Nayak, Debi P" uniqKey="Nayak D" first="Debi P." last="Nayak">Debi P. Nayak</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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